| 品牌 | 其他品牌 | 貨號 | A00186 |
|---|---|---|---|
| 規格 | 100ug | 供貨周期 | 一周 |
| 應用領域 | 生物產業 |
一、His抗體A00186產品介紹：
Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.
THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
中文：
特異于6個(ge)(ge)His標(biao)簽的單克隆抗體(ti)可以(yi)極大地提高(gao)幾(ji)種(zhong)不同(tong)免疫分析的有(you)效(xiao)性，幫助研究(jiu)人員識(shi)別、檢測(ce)和純化細(xi)菌、昆蟲細(xi)胞和哺乳動物細(xi)胞中(zhong)的多His融合蛋(dan)白(bai)。然而(er)，由于6XHis標(biao)簽的免疫原性較差，因(yin)此需要與KLH或其(qi)他(ta)載體(ti)結合作為(wei)免疫原。經(jing)過數百個(ge)(ge)選擇周期后(hou)，GenScript的研究(jiu)人員成功地分離出一種(zhong)針對他(ta)的標(biao)簽的抗體(ti)。
這(zhe)個™ His標(biao)記抗(kang)體(ti)mAb與小(xiao)鼠(shu)（IgG1亞型）具有很高的親和力。在GenScript上進行(xing)的測(ce)試顯示抗(kang)體(ti)也能識別(bie)4xHis和5xHis標(biao)簽。這(zhe)意味著(zhu)即使6xHis標(biao)簽僅部分暴露，它(ta)仍將(jiang)被該(gai)抗(kang)體(ti)識別(bie)和結合(he)。
THTM His標(biao)(biao)記單克(ke)隆(long)抗體由小(xiao)鼠腹水制備，并通過蛋(dan)白(bai)A親和(he)(he)柱(zhu)純化(hua)。該(gai)抗體識(shi)別天然和(he)(he)變性(xing)形(xing)式(shi)的合成多His和(he)(he)多His標(biao)(biao)記的融合蛋(dan)白(bai)。該(gai)產物與細(xi)菌、昆(kun)蟲細(xi)胞和(he)(he)哺乳(ru)動物細(xi)胞中(zhong)表達的融合蛋(dan)白(bai)反應。THTM-His標(biao)(biao)簽單克(ke)隆(long)抗體識(shi)別位(wei)于融合蛋(dan)白(bai)N端(duan)、C端(duan)和(he)(he)內部區(qu)域的His標(biao)(biao)簽。
THTM-His標記單抗可用于培養細胞的Western印跡分析、斑點印跡分析、ELISA、免疫熒光染色和流式細胞術。
二、His抗體A00186產品特性：
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
三、產品應用：
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
四、公司介紹：
漠(mo)寶(bao)（廈門）生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)科(ke)技有限公司(si)(si)是(shi)一家致(zhi)力(li)于生(sheng)(sheng)(sheng)(sheng)命(ming)科(ke)學(xue)和生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)技術領(ling)域(yu)的(de)企業，由廈門大學(xue)研究生(sheng)(sheng)(sheng)(sheng)創(chuang)辦。公司(si)(si)主要專注于生(sheng)(sheng)(sheng)(sheng)命(ming)科(ke)學(xue)科(ke)研試劑、耗材(cai)、儀器等科(ke)研用品的(de)開發與銷售(shou)。公司(si)(si)主要代(dai)理(li)歐美著名生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)技術公司(si)(si)的(de)產品，能為(wei)生(sheng)(sheng)(sheng)(sheng)命(ming)科(ke)學(xue)多研究領(ling)域(yu)的(de)科(ke)研人員提供全面的(de)產品。我們相信(xin)經(jing)過幾年的(de)努力(li)，漠(mo)寶(bao)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)一定能成為(wei)分(fen)子生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)學(xue)、細胞生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)學(xue)、免疫學(xue)等領(ling)域(yu)科(ke)研產品的(de)供應商。
漠(mo)寶(bao)生(sheng)物將(jiang)以更好的(de)客戶(hu)服務、完備的(de)產品供應、深厚的(de)專業知識(shi)和(he)優越的(de)人(ren)脈資源(yuan)，在同行業中保(bao)持良好的(de)企業形象和(he)競爭優勢。我們將(jiang)不(bu)斷(duan)引進優質的(de)產品，滿(man)足廣大客戶(hu)的(de)需求，在世界各國大力(li)(li)發展生(sheng)命科(ke)(ke)(ke)學(xue)的(de)時代背景下(xia)，我們將(jiang)以為“中國生(sheng)命科(ke)(ke)(ke)學(xue)發展而盡上一份綿薄之力(li)(li)"為使命，為科(ke)(ke)(ke)學(xue)人(ren)員創(chuang)造的(de)科(ke)(ke)(ke)研成果提供強(qiang)有力(li)(li)的(de)支撐。
展(zhan)望(wang)：公司將(jiang)借助于廈門大學細胞應(ying)激生物學國家重點實驗室的人(ren)才和(he)技(ji)術(shu)優勢(shi)，致力于研發(fa)、生產出品質(zhi)優越的生命(ming)科學研究中用到的試(shi)(shi)劑、抗體、試(shi)(shi)劑盒(he)等，力爭在不久的將(jiang)來將(jiang)漠寶打造為(wei)生命(ming)科學領域的。
公司部分(fen)代理品牌--Genscript（金斯瑞）、OriCell（賽(sai)業）、Affinibody（親和生命）、SmartBuffers（易優生物）、eppendorf（艾本德）、ALLSHENG(奧盛)、賽澳美（CellMax）、華盛源、珠海愷瑞、簡石生物、AMOBIO、Cohesion、CSNpharm、SAB、PPL、ZYMO RESEARCH等。
