| 品牌 | 其他品牌 | 貨號 | A00186 |
|---|---|---|---|
| 規格 | 100ug | 供貨周期 | 一周 |
| 應用領域 | 生物產業 |
THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞選(xuan)擇穩定(ding)、高產的雜交瘤細胞克隆(long)，然后(hou)使用相應(ying)的抗(kang)原進行篩選(xuan)分離(li)，制備出特(te)異性高、親和力強的THE™ 系列(lie)金牌(pai)抗(kang)體。
您(nin)在實驗(yan)中(zhong)是否曾經(jing)因為樣品量小、標(biao)簽只有部分暴露、融合蛋白變性或其它棘(ji)手的問題而感(gan)到煩惱？金(jin)斯瑞THE™ 系列金(jin)牌抗體，讓您(nin)的這些煩惱迎(ying)刃而解！
同時，我們也為您提供其它優質的(de)免疫(yi)學(xue)相(xiang)關產品。如憑(ping)借先進(jin)的(de)多肽合成能(neng)力，我們能(neng)夠(gou)提供大多數抗體(ti)的(de)封閉肽，讓(rang)您確認觀察到的(de)信號是由抗體(ti)與靶抗原之間的(de)相(xiang)互作用產生，而(er)不是由于(yu)非特異性結合而(er)產生。
靈敏(min)度—比市場上其他暢銷(xiao)的抗體更靈敏(min)；
特異性(xing)—無交叉反應；
親和力—-低解離常數Kd；
識(shi)別能(neng)力—能(neng)夠(gou)識(shi)別并結合廣泛的(de)物種及表達系(xi)統的(de)靶抗原；
低背(bei)景—無其它反應(ying)背(bei)景；
便(bian)捷—適用(yong)范圍廣(guang)，產品全面；
穩定—-好的批次間(jian)一致性，凍干(gan)狀(zhuang)態下，可至少穩定保存2年。
Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
公(gong)司介紹
漠寶（廈門）生(sheng)(sheng)(sheng)物(wu)科(ke)(ke)技有(you)限公司是一家致力(li)于(yu)生(sheng)(sheng)(sheng)命科(ke)(ke)學和生(sheng)(sheng)(sheng)物(wu)技術領(ling)(ling)域的企(qi)業，由廈門大學研(yan)究生(sheng)(sheng)(sheng)創辦(ban)。公司主要專(zhuan)注于(yu)生(sheng)(sheng)(sheng)命科(ke)(ke)學科(ke)(ke)研(yan)試(shi)劑、耗(hao)材(cai)、儀器(qi)等科(ke)(ke)研(yan)用品(pin)的開發與(yu)銷售。公司主要代理歐美著名生(sheng)(sheng)(sheng)物(wu)技術公司的產品(pin)，能(neng)為(wei)(wei)生(sheng)(sheng)(sheng)命科(ke)(ke)學多研(yan)究領(ling)(ling)域的科(ke)(ke)研(yan)人員提供全(quan)面的產品(pin)。我們相信經過幾年的努力(li)，漠寶生(sheng)(sheng)(sheng)物(wu)一定(ding)能(neng)成為(wei)(wei)分子生(sheng)(sheng)(sheng)物(wu)學、細胞生(sheng)(sheng)(sheng)物(wu)學、免(mian)疫學等領(ling)(ling)域科(ke)(ke)研(yan)產品(pin)的供應商。
漠寶生物將以更好的客戶服務、完備的產品供應、深厚的專業知識和優越的人脈資源，在同行業中保持良好的企業形象和競爭優勢。我們將不斷引進優質的產品，滿足廣大客戶的需求，在世界各國大力發展生命科學的時代背景下，我們將以“為中國生命科學發展而盡上一份綿薄之力"為使命，為科學人員創造的科研成果提供強有力的支撐。
展望：公(gong)司將借助于廈門大學(xue)細胞應(ying)激生物學(xue)國家(jia)重點實驗室的(de)人才和技術優(you)(you)勢，致(zhi)力(li)于研發、生產出品質(zhi)優(you)(you)越的(de)生命(ming)科學(xue)研究中用(yong)到的(de)試劑(ji)、抗體、試劑(ji)盒等，力(li)爭在不久的(de)將來將漠寶(bao)打造為生命(ming)科學(xue)領域(yu)的(de)。
公司(si)部分代理品牌--Genscript（金斯瑞）、OriCell（賽(sai)(sai)業）、Affinibody（親和(he)生(sheng)命）、SmartBuffers（易(yi)優(you)生(sheng)物）、eppendorf（艾本(ben)德）、ALLSHENG(奧盛)、賽(sai)(sai)澳美（CellMax）、華盛源、珠海愷瑞、簡石生(sheng)物、AMOBIO、Cohesion、CSNpharm、SAB、PPL、ZYMO RESEARCH等。
